Abstract
Basophils play a key role in the orientation of immune responses. Though the interaction of SARS-CoV-2 with various immune cells has been relatively well studied, the response of basophils to this pandemic virus is not characterized yet. In this study, we report that SARS-CoV-2 induces cytokine responses and in particular IL-13, in both resting and IL-3 primed basophils. The response was prominent under IL-3 primed condition. However, either SARS-CoV-2 or SARS-CoV-2-infected epithelial cells did not alter the expression of surface markers associated with the activation of basophils, such as CD69, CD13 and/or degranulation marker CD107a. We also validate that human basophils are not permissive to SARS-CoV-2 replication. Though increased expression of immune checkpoint molecule PD-L1 has been reported on the basophils from COVID-19 patients, we observed that SARS-CoV-2 does not induce PD-L1 on the basophils. Our data suggest that basophil cytokine responses to SARS-CoV-2 might help in reducing the inflammation and also to promote antibody responses to the virus.
Keywords: basophils; COVID-19; Caco-2 cells; IL-13; IL-3; IL-4; SARS-CoV-2; epithelial cells.
【저자키워드】 COVID-19, SARS-CoV-2, epithelial cells, basophils, Caco-2 cells, IL-13, IL-3, IL-4, 【초록키워드】 Inflammation, Antibody Response, cytokine, virus, immune, PD-L1, Antibody responses, immune responses, immune cells, epithelial cells, expression, SARS-CoV-2 replication, COVID-19 patients, marker, basophils, Caco-2 cells, IL-13, IL-4, basophil, Interaction, Immune cell, CD69, cytokine response, Caco-2, epithelial cell, Orientation, Activation, increased expression, help, CD107a, pandemic virus, degranulation, surface markers, Alter, CD13, reported, characterized, reducing, promote, induce, surface marker, 【제목키워드】 Human, response, basophil,