Abstract
The COVID-19 emergency pandemic resulting from infection with SARS-CoV-2 represents a major threat to public health worldwide. There is an urgent clinical demand for easily accessible tools to address weaknesses and gaps in the management of COVID-19 patients. In this context, transcriptomic profiling of liquid biopsies, especially microRNAs (miRNAs), has recently emerged as a robust source of potential clinical indicators for medical decision-making. Nevertheless, the analysis of the circulating miRNA signature and its translation to clinical practice requires strict control of a wide array of methodological details. In this review, we indicate the main methodological aspects that should be addressed when evaluating the circulating miRNA profiles in COVID-19 patients, from preanalytical and analytical variables to the experimental design, impact of confounding, analysis of the data and interpretation of the findings, among others. Additionally, we provide practice points to ensure the rigour and reproducibility of miRNA-based biomarker investigations of this condition. Abbreviations: ACE: angiotensin-converting enzyme; ARDS: acute respiratory distress syndrome; COVID-19: coronavirus disease 2019; ERDN: early Detection Research Network; LMWH: low molecular weight heparin; miRNA: microRNA; ncRNA: noncoding RNA; SARS-CoV-2: severe acute respiratory syndrome coronavirus-2; SOP: standard operating procedure.
Keywords: Blood; COVID-19; RT-qPCR; SARS-CoV-2; biomarker; liquid biopsy; methods; microRNA; noncoding RNA.
【저자키워드】 COVID-19, SARS-CoV-2, Biomarker, microRNA, RT-qPCR, noncoding RNA, Methods, Blood, liquid biopsy, 【초록키워드】 coronavirus disease, public health, Coronavirus disease 2019, ARDS, coronavirus, pandemic, Biomarker, acute respiratory distress syndrome, translation, miRNA, detection, heparin, severe acute respiratory syndrome Coronavirus, Severe acute respiratory syndrome, RT-qPCR, miRNAs, biopsies, qPCR, management, Early detection, Interpretation, noncoding RNA, respiratory, MicroRNAs, COVID-19 patients, Clinical practice, Angiotensin-converting enzyme, acute respiratory distress, Analysis, angiotensin, low molecular weight, respiratory distress, low molecular weight heparin, acute respiratory syndrome, among others, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus-2, circulating, miRNA signature, reproducibility, COVID-19 emergency, LMWH, variable, experimental design, ncRNA, transcriptomic, circulating miRNA profiles, rigour, robust, resulting, addressed, methodological, circulating miRNA profile, infection with SARS-CoV-2, 【제목키워드】 peripheral, methodological,