Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.
Keywords: hamster lung; hsa-miR-15b-5p; hsa-miR-195-5p; microRNA; severe acute respiratory syndrome coronavirus 2.
【저자키워드】 microRNA, Severe acute respiratory syndrome coronavirus 2., hamster lung, hsa-miR-15b-5p, hsa-miR-195-5p, 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Coronavirus disease 2019, ACE2, TMPRSS2, coronavirus, Pneumonia, SARS-CoV, SARS-COV-2 infection, microRNA, miRNA, Infection, lung, Infectious disease, MERS, severe acute respiratory syndrome Coronavirus, cough, MERS-CoV, miRNAs, Fever, Dyspnea, Research, ADAM17, respiratory, expression, MicroRNAs, binding, Diagnostic biomarkers, genome sequence, Genome sequences, Middle East, acute respiratory syndrome, differential expression, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, severe symptoms, lung tissue, hsa-miR-15a-5p, miR-195-5p, receptor proteins, lung tissues, expression patterns, expression pattern, infection mechanism, diagnostic biomarker, examined, caused, lung;, 【제목키워드】 SARS-CoV-2, hamster,