Abstract
Coronavirus disease 2019, a newly emerging serious infectious disease, has spread worldwide. To date, effective drugs against the disease are limited. Traditional Chinese medicine was commonly used in treating COVID-19 patients in China. Here we tried to identify herbal effective lipid compounds from the lipid library of 92 heat-clearing and detoxication Chinese herbs. Through virtual screening, enzymatic activity and inhibition assays, and surface plasmon resonance tests, we identified lipid compounds targeting the main protease (M pro ) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and verified their functions. Here, we found that natural lipid compounds LPC (14:0/0:0) and LPC (16:0/0:0) could target SARS-CoV-2 M pro , recover cell death induced by SARS-CoV-2, and ameliorate acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) induced by bacterial lipopolysaccharides and virus poly (I:C) mimics in vivo and in vitro. Our results suggest that LPC (14:0/0:0) and LPC (16:0/0:0) might be potential pan remedy against ARDS.
Keywords: ALI/ARDS; Mpro; SARS-CoV-2; pan-anti-inflammatory; traditional Chinese medicine.
【저자키워드】 SARS-CoV-2, traditional Chinese medicine., MPro, ALI/ARDS, pan-anti-inflammatory, 【초록키워드】 coronavirus disease, Respiratory distress syndrome, Coronavirus disease 2019, ARDS, coronavirus, acute respiratory distress syndrome, Anti-inflammatory, Traditional Chinese medicine, Virtual screening, Infectious disease, Lung injury, protease, in vitro, surface plasmon resonance, severe acute respiratory syndrome Coronavirus, virus, acute lung injury, effective drugs, MPro, Spread, China, in vivo, cell death, COVID-19 patients, distress, respiratory distress, lipopolysaccharides, acute respiratory syndrome, surface plasmon, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, enzymatic activity, Compound, M pro, syndrome, Chinese, functions, Inhibition assays, bacterial lipopolysaccharide, effective, identify, the disease, effective drug, treating COVID-19 patient, 【제목키워드】 mice, Herbal, inhibitors of SARS-CoV-2,