Abstract
SARS-CoV-2 is a highly virulent coronavirus that first surfaced in late 2019 and has since created a pandemic of the acute respiratory sickness known as “coronavirus disease 2019” (COVID-19), posing a threat to human health and public safety. S-RBD is a coronaviral protein that is essential for a coronavirus (CoV) to bind and penetrate into host cells. As a result, it has become a popular pharmacological target. The goal of this study was to find potential candidates for anti-coronavirus disease 2019 (COVID-19) drugs by targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S-RBD with novel bioactive compounds and molecular interaction studies of 15,000 phytochemicals belonging to different flavonoid subgroups. A spike protein crystal structure attached to the ACE2 structure was obtained from the PDB database. A library of 15,000 phytochemicals was made by collecting compounds from different databases, such as the Zinc-database, PubChem-database, and MPD3-database. This library was docked against a receptor binding domain of a spike glycoprotein through the Molecular Operating Environment (MOE). The top drug candidates Phylloflavan, Milk thistle, Ilexin B and Isosilybin B, after virtual screening, were selected on the basis of the least binding score. Phylloflavan ranked as the top compound because of its least binding affinity score of -14.09 kcal/mol. In silico studies showed that all those compounds showed good activity and could be used as an immunological response with no bioavailability issues. Absorption, distribution, metabolism, excretion and a toxicological analysis were conducted through SwissADME. Stability and effectiveness of the docked complexes were elucidated by performing the 100 ns molecular dynamic simulation through the Desmond package.
Keywords: SARS-CoV-2; drug targets; molecular docking; molecular dynamic simulation; phytochemicals.
【저자키워드】 SARS-CoV-2, molecular docking, drug targets, molecular dynamic simulation, phytochemicals., 【초록키워드】 COVID-19, coronavirus disease, Zinc, Coronavirus disease 2019, ACE2, coronavirus, pandemic, spike glycoprotein, molecular docking, Virtual screening, drug, severe acute respiratory syndrome Coronavirus, database, metabolism, binding affinity, Spike protein, Environment, Receptor binding domain, Bioavailability, Protein, Health, flavonoid, S-RBD, Effectiveness, CoV, molecular, crystal structure, distribution, disease, binding, Immunological response, Analysis, host cells, acute respiratory syndrome, Sickness, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, subgroups, PubChem, Compound, candidate, milk, library, silico studies, Absorption, excretion, drug candidate, molecular interaction, docked complexes, Coronaviral, virulent, pharmacological target, PDB, MPD3, Phylloflavan, thistle, selected, conducted, was obtained, docked, docked complex, penetrate, silico study, 【제목키워드】 spike, drug, natural, phytochemical,