Abstract
The SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) is essential for virus replication, therefore it is a promising drug target. Here we present a surface plasmon resonance approach to study the interaction of RdRp with drugs in real time. We monitored the effect of favipiravir, ribavirin, sofosbuvir triphosphate PSI-7409 and suramin on RdRp binding to RNA immobilized on the chip. Suramin precluded interaction of RdRp with RNA and even displaced RdRp from RNA.
Keywords: RNA-dependent RNA polymerase; SARS-CoV-2; Suramin; Surface plasmon resonance.
All Keywords
【저자키워드】 SARS-CoV-2, surface plasmon resonance, RNA-dependent RNA polymerase, Suramin, 【초록키워드】 Favipiravir, drug, surface plasmon resonance, ribavirin, sofosbuvir, RNA, drug target, RdRP, RNA-dependent RNA polymerase, virus replication, SARS-CoV-2 RNA, RNA polymerase, binding, Suramin, Interaction, real time, surface plasmon, ChIP, approach, precluded, 【제목키워드】 Treatment, Suramin, Interaction, study drug, approach, highlight,
【저자키워드】 SARS-CoV-2, surface plasmon resonance, RNA-dependent RNA polymerase, Suramin, 【초록키워드】 Favipiravir, drug, surface plasmon resonance, ribavirin, sofosbuvir, RNA, drug target, RdRP, RNA-dependent RNA polymerase, virus replication, SARS-CoV-2 RNA, RNA polymerase, binding, Suramin, Interaction, real time, surface plasmon, ChIP, approach, precluded, 【제목키워드】 Treatment, Suramin, Interaction, study drug, approach, highlight,
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SARS-CoV-2 RNA 의존성 RNA 중합효소(RdRp)는 바이러스 복제에 필수적이므로 유망한 약물 표적입니다. 여기에서 실시간으로 약물과 RdRp의 상호 작용을 연구하기 위해 표면 플라즈몬 공명 접근 방식을 제시합니다. 우리는 칩에 고정된 RNA에 대한 RdRp 결합에 대한 favipiravir, ribavirin, sofosbuvir triphosphate PSI-7409 및 suramin의 효과를 모니터링했습니다. Suramin은 RdRp와 RNA의 상호 작용을 배제하고 RNA에서 RdRp를 대체했습니다.
{{ 키워드: }} RNA 의존성 RNA 중합효소; 사스 코로나바이러스 2; 수라민; 표면 플라즈몬 공명.