Abstract
We evaluated the SARS-CoV-2-inactivation activity of ozonated glycerol (OG). When a viral solution with 1% fetal bovine serum (FBS) was mixed with test solutions at a ratio of 1:19 and incubated for 20 s, OG with ozone concentrations of over 1000 ppm inactivated ≥ 94.38% of the virus. Extension of the reaction time to 1 h led to the inactivation of ≥ 99.82% of the virus (the viral titer was below the detection limit). Extension to 24 h resulted in concentrations over 200 ppm OG inactivating ≥ 99.87% of the virus (the viral titers were below the detection limit). Next, viral solutions with 1, 20, and 40% FBS were mixed with test solutions at a ratio of 1:19 and incubated for 5 min. Whereas the virucidal activity of 500 ppm OG was very limited in the presence of 1% FBS (79.47% inactivation), it increased in the presence of 20 and 40% FBS (95.13 and 97.95% inactivation, respectively; the viral titers were not below the detection limit). Meanwhile, over 1000 ppm OG inactivated ≥ 99.44% of the virus regardless of the FBS concentration (the viral titers were below the detection limit). Extension of the reaction time to 1 h led to 500 ppm OG inactivating ≥ 99.91 and ≥ 99.95% of the virus with 20 and 40% FBS, respectively (the viral titers were below the detection limit). These results suggested that OG might be useful as a virucidal agent against SARS-CoV-2.
Keywords: Antiviral material; Hand hygiene; Ozonated glycerol; Severe acute respiratory syndrome coronavirus 2.
【저자키워드】 severe acute respiratory syndrome coronavirus 2, hand hygiene, Antiviral material, Ozonated glycerol, 【초록키워드】 severe acute respiratory syndrome coronavirus 2, SARS-CoV-2, coronavirus, severe acute respiratory syndrome Coronavirus, virus, serum, inactivation, virucidal, bovine, hand hygiene, inactivated, Ozone, Detection limit, Concentration, PPM, acute respiratory syndrome, fetal bovine serum, reaction time, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, hand, viral titers, extension, glycerol, viral titer, evaluated, suggested, fetal, incubated, FBS, 【제목키워드】 SARS-CoV-2,