Abstract
We introduce a target capture next-generation sequencing methodology, the ONETest Coronaviruses Plus, to sequence the SARS-CoV-2 genome and select loci of other respiratory viruses. We applied the ONETest on 70 respiratory samples (collected in Florida, USA between May and July, 2020), in which SARS-CoV-2 had been detected by a PCR assay. For 48 of the samples, we also applied the ARTIC protocol. Of the 70 ONETest libraries, 45 (64%) had a (near-)complete sequence (>29,000 bases and >90% covered by >9 reads). Of the 48 ARTIC libraries, 25 (52%) had a (near-)complete sequence. In 19 out of 25 (76%) samples in which both the ONETest and ARTIC yielded (near-)complete sequences, the lineages assigned were identical. As a target capture approach, the ONETest is less prone to loss of sequence coverage than amplicon approaches, and thus can provide complete genomic information more often to track and monitor SARS-CoV-2 variants.
Keywords: COVID-19; Genome sequencing; Respiratory viruses; Target hybridization.
【저자키워드】 COVID-19, respiratory viruses, Genome sequencing, Target hybridization., 【초록키워드】 SARS-CoV-2, respiratory viruses, Genome sequencing, Coverage, SARS-CoV-2 variants, SARS-CoV-2 genome, Next-generation sequencing, Lineage, target, methodology, respiratory, USA, lineages, ARTIC protocol, Other respiratory viruses, approaches, sequence, PCR assay, base, loci, bases, MONITOR, genomic information, Complete, approach, respiratory sample, collected, applied, less, assigned, the SARS-CoV-2 genome, 【제목키워드】 target, capture sequencing,