Abstract
The development of rapid, highly sensitive, and selective methods for the diagnosis of infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) should help to prevent the spread of this pandemic virus. In this study, we combined recombinase polymerase amplification (RPA), as a means of isothermal DNA amplification, with an rkDNA-graphene oxide (GO) probe system to allow the rapid detection of SARS-CoV-2 with high sensitivity and selectivity. We used in situ enzymatic synthesis to prepare an rkDNA probe that was complementary to an RPA-amplified sequence of the target N-gene of SARS-CoV-2. The fluorescence of this rkDNA was perfectly quenched in the presence of GO. When the quenched rkDNA-GO system was added to the RPA-amplified sequence of the target SARS-CoV-2, the fluorescence recovered dramatically. The combined RPA/rkDNA-GO system exhibited extremely high selectivity (discrimination factor: 17.2) and sensitivity (LOD = 6.0 aM) for the detection of SARS-CoV-2. The total processing time was only 1.6 h. This combined RPA/rkDNA-GO system appears to be a very efficient and simple method for the point-of-care detection of SARS-CoV-2.
Keywords: Enzymatic in situ synthetic probe (rkDNA); Fluorescence; Graphene oxide; Isothermal amplification; RPA; SARS-CoV-2.
【저자키워드】 SARS-CoV-2, Fluorescence, isothermal amplification, RPA, Enzymatic in situ synthetic probe (rkDNA), Graphene oxide, 【초록키워드】 SARS-CoV-2, coronavirus, Infection, Diagnosis, severe acute respiratory syndrome Coronavirus, Spread, point-of-care, amplification, sensitivity, Fluorescence, target, isothermal amplification, selectivity, Graphene, isothermal, complementary, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, sequence, help, selective, N-gene, DNA amplification, polymerase, pandemic virus, probe, Prevent, exhibited, appear, added, 【제목키워드】 SARS-CoV-2, polymerase, Combined,