Abstract
Considering the worldwide health crisis associated with highly contagious severe respiratory disease of COVID-19 outbreak, the development of multiplexed, simple and rapid diagnostic platforms to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is in high demand. Here, a nucleic acid amplification-free electrochemical biosensor based on four-way junction (4-WJ) hybridization is presented for the detection of SARS-CoV-2. To form a 4-WJ structure, a Universal DNA-Hairpin (UDH) probe is hybridized with two adaptor strands and a SARS-CoV-2 RNA target. One of the adaptor strands is functionalized with a redox mediator that can be detected using an electrochemical biosensor. The biosensor could simultaneously detect 5.0 and 6.8 ag/μL of S and Orf1ab genes, respectively, within 1 h. The biosensor was evaluated with 21 clinical samples (16 positive and 5 negative). The results revealed a satisfactory agreement with qRT-PCR. In conclusion, this biosensor has the potential to be used as an on-site, real-time diagnostic test for COVID-19.
Keywords: COVID-19; Electrochemical biosensor; Orf1ab gene; S gene; SARS-CoV-2 RNA.
【저자키워드】 COVID-19, SARS-CoV-2 RNA, electrochemical biosensor, ORF1ab gene, S gene, 【초록키워드】 SARS-CoV-2, coronavirus, qRT-PCR, diagnostic test, diagnostic, severe acute respiratory syndrome Coronavirus, Health crisis, biosensor, nucleic acid, Health, COVID-19 outbreak, Respiratory disease, SARS-CoV-2 RNA, nucleic acid amplification, platform, ORF1ab gene, S gene, mediator, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, severe respiratory disease, positive, contagious, probe, Genes, clinical sample, detect, hybridized, evaluated, Universal, 【제목키워드】 Rapid,