Abstract
The clinical symptoms caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are nonspecific and can be associated with most other respiratory viruses that cause acute respiratory tract infections (ARI). Because the clinical differentiation of COVID-19 patients from those with other respiratory viruses is difficult, the evaluation of automated methods to detect important respiratory viruses together with SARS-CoV-2 seems necessary. Therefore, this study compares two molecular assays for the detection of respiratory viruses, including SARS-CoV-2: the Respiratory Viruses 16-Well Assay (AusDiagnostics, Pty Ltd., Mascot, Australia) and the Allplex™ RV Essential Assay coupled with the Allplex™-nCoV Assay (Seegene Inc., Seoul, Korea). The two methods (AusDiagnostics and Alplex TM -nCoV Assay SARS-CoV-2) had 98.6% agreement with the reference method, cobas 6800, for the detection of SARS-CoV-2. Agreement between the AusDiagnostics assay and the Alplex TM RV Essential Assay for the detection of seven respiratory viruses was 99%. In our experience, the Respiratory Viruses 16-Well Assay proved to be the most valuable and useful medium-throughput method for simultaneous detection of important respiratory viruses and SARS-CoV-2. The main advantages of the method are high specificity for all targets included and their simultaneous detection and medium throughput with the option of having multiple instruments provide a constant run.
Keywords: COVID-19; SARS-CoV-2; molecular assay; respiratory viruses; validation.
【저자키워드】 COVID-19, SARS-CoV-2, respiratory viruses, validation, Molecular assay, 【초록키워드】 coronavirus, Clinical symptoms, respiratory tract infection, severe acute respiratory syndrome Coronavirus, respiratory viruses, specificity, ARI, respiratory virus, target, automated, agreement, molecular, respiratory, COVID-19 patients, Other respiratory viruses, COVID-19 patient, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, medium, clinical symptom, option, reference method, AusDiagnostics, AusDiagnostics assay, Mascot, Seven, detect, caused, other respiratory virus, Essential, Inc., nonspecific, 【제목키워드】 detection, target, respiratory, New,