Abstract
A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was applied. The AuPs deposited on the electrode were involved in a complexation reaction with creatinine, resulting in a decrease in the analytical response, and thus providing a fast and simple electroanalytical device. Physicochemical characterizations were performed by SEM, EIS, FTIR, and cyclic voltammetry. Square wave voltammetry was employed for the creatinine detection, and the sensor presented a linear response with a detection limit of 0.016 mmol L -1 . Finally, a biosensor for the detection of SARS-CoV-2 was developed based on the immobilization of a capture sequence of the viral cDNA upon the Au-modified 3D-printed electrode. The concentration, immobilization time, and hybridization time were evaluated in presence of the DNA target, resulting in a biosensor with rapid and low-cost analysis, capable of sensing the cDNA of the virus with a good limit of detection (0.30 µmol L -1 ), and high sensitivity (0.583 µA µmol -1 L). Reproducible results were obtained (RSD = 1.14%, n = 3), attesting to the potentiality of 3D-printed platforms for the production of biosensors.
Keywords: 3D printed electrode; AuP modified electrode; SARS-CoV-2; creatinine; electrochemical (bio)sensor.
【저자키워드】 SARS-CoV-2, 3D printed electrode, AuP modified electrode, creatinine, electrochemical (bio)sensor., 【초록키워드】 COVID-19, Biomarker, virus, Particle, biosensor, sensor, DNA, sensitivity, limit of detection, gold, immobilization, platform, Graphene, Detection limit, creatinine, Concentration, Analysis, SEM, cDNA, sequence, potentiality, decrease, electrode, viral cDNA, Square wave, resulting, performed, detect, involved, evaluated, linear, applied, 【제목키워드】 detection, SARS-CoV-2 cDNA,