Abstract
New platforms for the rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern are urgently needed. Here we report the development of a nanomechanical sensor based on the deflection of a microcantilever capable of detecting the SARS-CoV-2 spike (S) glycoprotein antigen using computationally designed multivalent minibinders immobilized on a microcantilever surface. The sensor exhibits rapid (<5 min) detection of the target antigens down to concentrations of 0.05 ng/mL (362 fM) and is more than an order of magnitude more sensitive than an antibody-based cantilever sensor. Validation of the sensor with clinical samples from 33 patients, including 9 patients infected with the Omicron (BA.1) variant observed detection of antigen from nasopharyngeal swabs with cycle threshold (Ct) values as high as 39, suggesting a limit of detection similar to that of the quantitative reverse transcription polymerase chain reaction (RT-qPCR). Our findings demonstrate the use of minibinders and nanomechanical sensors for the rapid and sensitive detection of SARS-CoV-2 and potentially other disease markers.
【초록키워드】 SARS-CoV-2, coronavirus, antibody, variant, variants of concern, severe acute respiratory syndrome Coronavirus, omicron, variants, Antigen, Nasopharyngeal swab, polymerase chain reaction, clinical samples, sensor, RT-qPCR, validation, cycle threshold, nasopharyngeal swabs, Patient, limit of detection, Rapid, reverse transcription, glycoprotein, disease, Quantitative, patients, platform, SARS-CoV-2 spike, Concentration, Chain Reaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, deflection, polymerase chain, clinical sample, magnitude, exhibit, New, the SARS-CoV-2, 【제목키워드】 SARS-CoV-2, detection, multivalent, sensitive, concern,