Abstract
The sudden increase of the COVID-19 outbreak and its continued growth with mutations in various forms has created a global health crisis as well as devastating social and economic effects over the past two years. In this study, a screen-printed carbon electrode reinforced with boron nitride quantum dots/flower-like gold nanostructures (BNQDs/FGNs/SPCE) and functionalized by highly specific antisense DNA oligonucleotide presents an alternative and promising solution for targeting SARS-CoV-2 RNA without nucleic acid amplification. The platform was tested on 120 SARS-CoV-2 RNA isolated from real clinical samples (60 positive and 60 negative confirmed by conventional RT-PCR method). Based on obtained quantitative results and statistical analysis (box-diagram, cutoff value, receiver operating characteristic curve, and t-test), the biosensor revealed a significant difference between the two positive and negative groups with 100% sensitivity and 100% specificity. To evaluate the quantitation capacity and detection limit of the biosensor for clinical trials, the detection performance of the biosensor for continuously diluted RNA isolated from SARS-CoV-2-confirmed patients was compared to those obtained by RT-PCR, demonstrating that the detection limit of the biosensor is lower than or comparable to that of RT-PCR. The ssDNA/BNQDs/FGNs/SPCE showed negligible cross-reactivity with RNA fragments isolated from Influenza A (IAV) clinical samples and also remained stable for up to 14 days. In conclusion, the fabricated biosensor may serve as a promising tool for point-of-care applications.
Keywords: COVID-19 pandemic; Electrochemical biosensor; SARS-CoV-2 RNA; Virus diagnosis.
【저자키워드】 COVID-19 pandemic, SARS-CoV-2 RNA, electrochemical biosensor, Virus diagnosis., 【초록키워드】 COVID-19, SARS-CoV-2, Mutation, Influenza, COVID-19 pandemic, Diagnosis, clinical trials, RT-PCR, influenza A, Health crisis, RNA, cross-reactivity, biosensor, point-of-care, clinical samples, nucleic acid, DNA, sensitivity, specificity, Health, Screen, COVID-19 outbreak, Patient, gold, SARS-CoV-2 RNA, nucleic acid amplification, group, characteristic, Quantitative, platform, Detection limit, statistical analysis, receiver operating characteristic, significant difference, growth, t-test, receiver operating characteristic curve, cutoff value, quantitation, positive, carbon, ssDNA, Effect, IAV, Boron, boron nitride, clinical sample, evaluate, remained, form, comparable, RNA fragment, was tested, 【제목키워드】 amplification, sensitive, clinically, diagnosis of SARS-CoV-2,