Abstract
Accurate and population-scale screening technology is crucial in the control and prevention of COVID-19, such as pooled testing with high overall testing efficiency. Nevertheless, pooled testing faces challenges in sensitivity and specificity due to diluted targets and increased contaminations. Here, we develop a graphene field-effect transistor sensor modified with triple-probe tetrahedral DNA framework (TDF) dimers for 10-in-1 pooled testing of SARS-CoV-2 RNA. The synergy effect of triple probes as well as the special nanostructure achieve a higher binding affinity, faster response, and better specificity. The detectable concentration reaches 0.025-0.05 copy μL -1 in unamplified samples, lower than that of the reverse transcript-polymerase chain reaction. Without a requirement of nucleic-acid amplification, the sensors identify all of the 14 positive cases in 30 nasopharyngeal swabs within an average diagnosis time of 74 s. Unamplified 10-in-1 pooled testing enabled by the triple-probe TDF dimer sensor has great potential in the screening of COVID-19 and other epidemic diseases.
Keywords: COVID-19; DNA nanostructure; field-effect transistor; pooled testing.
【저자키워드】 COVID-19, field-effect transistor, DNA nanostructure, pooled testing., 【초록키워드】 Diagnosis, binding affinity, Nasopharyngeal swab, polymerase chain reaction, amplification, DNA, specificity, Sensitivity and specificity, nasopharyngeal swabs, field-effect transistor, target, SARS-CoV-2 RNA, synergy, Graphene, Concentration, Efficiency, Chain Reaction, epidemic diseases, average, positive, probe, prevention of COVID-19, identify, develop, detectable, faster, 【제목키워드】 SARS-CoV-2, DNA, Testing, transistor,