Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for many pathological processes, including altered vascular disease development, dysfunctional thrombosis and a heightened inflammatory response. However, there is limited work to determine the underlying cellular responses induced by exposure to SARS-CoV-2 structural proteins. Thus, our objective was to investigate how human arterial adventitial fibroblasts inflammation, thrombosis and diabetic disease markers are altered in response to Spike, Nucleocapsid and Membrane-Envelope proteins. We hypothesized that after a short-term exposure to SARS-CoV-2 proteins, adventitial fibroblasts would have a higher expression of inflammatory, thrombotic and diabetic proteins, which would support a mechanism for altered vascular disease progression. After incubation, the expression of gC1qR, ICAM-1, tissue factor, RAGE and GLUT-4 was significantly up-regulated. In general, the extent of expression was different for each SARS-CoV-2 protein, suggesting that SARS-CoV-2 proteins interact with cells through different mechanisms. Thus, SARS-CoV-2 protein interaction with vascular cells may regulate vascular disease responses.
Keywords: COVID-19; Cardiovascular; Complement; Inflammation; SARS-CoV-2; Thrombosis.
【저자키워드】 COVID-19, SARS-CoV-2, Inflammation, complement, cardiovascular, thrombosis., 【초록키워드】 severe acute respiratory syndrome coronavirus 2, coronavirus, thrombosis, spike, RAGE, complement, ICAM-1, Proteins, progression, severe acute respiratory syndrome Coronavirus, nucleocapsid, structural proteins, mechanisms, Incubation, disease, expression, mechanism, cellular response, marker, Tissue Factor, Interaction, Inflammatory response, Inflammatory, regulate, SARS-CoV-2 proteins, exposure to, vascular disease, Support, Diabetic, acute respiratory syndrome, Vascular, acute respiratory syndrome coronavirus, tissue, thrombotic, SARS-CoV-2 protein, envelope proteins, fibroblast, responses, Cell, responsible, significantly, determine, up-regulated, 【제목키워드】 response, Inflammatory, regulate, fibroblast,