Abstract
We compared the perfomance of SARS-CoV-2 reverse transcriptase real-time polymerase chain reaction (RT-PCR) to droplet digital PCR (ddPCR). 95% and 40% of positive and negative RT-PCR specimens, respectively, were positive on ddPCR yielding sensitivities of 84% (95% CI: 74, 91) and 97% (95% CI: 89, 99), for RT-PCR and ddPCR, respectively. We found that SARS-CoV-2 RT-PCR testing in children has a concerning false-negative rate at lower nucleocapsid gene copy numbers.
All Keywords
【초록키워드】 SARS-CoV-2, children, RT-PCR, polymerase chain reaction, digital PCR, sensitivity, PCR testing, SARS-CoV-2 RT-PCR, false-negative rate, specimens, Nucleocapsid gene, reverse transcriptase, Chain Reaction, 95% CI, RT-PCR testing, sensitivities, positive, transcriptase, negative RT-PCR, polymerase chain, concerning, yielding, 【제목키워드】 SARS-CoV-2, clinical, Prospective, Emergency, load, finding, Department,
【초록키워드】 SARS-CoV-2, children, RT-PCR, polymerase chain reaction, digital PCR, sensitivity, PCR testing, SARS-CoV-2 RT-PCR, false-negative rate, specimens, Nucleocapsid gene, reverse transcriptase, Chain Reaction, 95% CI, RT-PCR testing, sensitivities, positive, transcriptase, negative RT-PCR, polymerase chain, concerning, yielding, 【제목키워드】 SARS-CoV-2, clinical, Prospective, Emergency, load, finding, Department,
{{{ 추상적인 }}}
우리는 SARS-CoV-2 역전사효소 실시간 중합효소 연쇄 반응(RT-PCR)의 성능을 물방울 디지털 PCR(ddPCR)과 비교했습니다. 양성 및 음성 RT-PCR 표본의 각각 95% 및 40%는 ddPCR에서 양성이었고 RT-PCR의 경우 84%(95% CI: 74, 91) 및 97%(95% CI: 89, 99)의 감도를 생성했습니다. 각각 PCR 및 ddPCR. 우리는 어린이를 대상으로 한 SARS-CoV-2 RT-PCR 검사가 더 낮은 뉴클레오캡시드 유전자 카피 수에서 우려되는 위음성 비율을 갖는다는 것을 발견했습니다.