Abstract
Coronavirus disease 19 (COVID-19) clinical manifestations include the involvement of the gastrointestinal tract, affecting around 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected children. In the present work, the consequence of a short time of viral absorption (5, 15, 30 and 60 min) was tested on the Caco-2 intestinal epithelial cell line. Our findings show that Caco-2 cells are highly permissive to SARS-CoV-2 infection, even after 5 min of viral inoculation at a multiplicity of infection of 0.1. No cytopathic effect was evident during the subsequent 7 days of monitoring; nevertheless, the immunofluorescence staining for the viral nucleocapsid confirmed the presence of intracellular SARS-CoV-2. Our findings highlight the very short time during which SARS-CoV-2 is able to infect these cells in vitro.
Keywords: Caco-2 cell line; SARS-CoV-2; short time of infection; viral absorption.
【저자키워드】 SARS-CoV-2, Caco-2 cell line, short time of infection, viral absorption., 【초록키워드】 COVID-19, coronavirus disease, coronavirus, SARS-COV-2 infection, children, in vitro, severe acute respiratory syndrome Coronavirus, clinical manifestations, immunofluorescence, coronavirus disease 19, gastrointestinal tract, Caco-2 cells, Cytopathic effect, Caco-2, epithelial cell, clinical manifestation, multiplicity of infection, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, These cells, cell line, multiplicity, staining, immunofluorescence staining, infect, Cell, intestinal, highlight, include, subsequent, epithelial cell line, affecting, these cell, the viral nucleocapsid, Caco-2 cell, was tested, 【제목키워드】 Effect, time,