Abstract
Coronavirus disease 2019 (COVID-19) remains a global public health threat. Hence, more effective and specific antivirals are urgently needed. Here, COVID-19 hyperimmune globulin (COVID-HIG), a passive immunotherapy, is prepared from the plasma of healthy donors vaccinated with BBIBP-CorV (Sinopharm COVID-19 vaccine). COVID-HIG shows high-affinity binding to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein, the receptor-binding domain (RBD), the N-terminal domain of the S protein, and the nucleocapsid protein; and blocks RBD binding to human angiotensin-converting enzyme 2 (hACE2). Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro. However, a significant reduction in the neutralization titer is detected against Beta, Delta, and Omicron variants. Additionally, assessments of the prophylactic and treatment efficacy of COVID-HIG in an Adv5-hACE2-transduced IFNAR -/- mouse model of SARS-CoV-2 infection show significantly reduced weight loss, lung viral loads, and lung pathological injury. Moreover, COVID-HIG exhibits neutralization potency similar to that of anti-SARS-CoV-2 hyperimmune globulin from pooled convalescent plasma. Overall, the results demonstrate the potential of COVID-HIG against SARS-CoV-2 infection and provide reference for subsequent clinical trials.
Keywords: COVID-19 hyperimmune globulin; SARS-CoV-2 variant; passive immunotherapy; sinopharm COVID-19 vaccine.
【저자키워드】 SARS-CoV-2 variant, Passive immunotherapy, COVID-19 hyperimmune globulin, sinopharm COVID-19 vaccine., 【초록키워드】 COVID-19, Treatment, coronavirus disease, convalescent plasma, SARS-CoV-2, Coronavirus disease 2019, Efficacy, coronavirus, COVID-19 vaccine, Antiviral, S protein, B.1.351, neutralization, Immunotherapy, SARS-COV-2 infection, SARS-CoV-2 variant, Delta, B.1.617.2, lung, clinical trials, in vitro, Prophylactic, severe acute respiratory syndrome Coronavirus, omicron, anti-SARS-CoV-2, Omicron variants, variants, hACE2, nucleocapsid protein, Protein, Receptor-binding domain, SARS-CoV-2 variants, B.1.1.7, nucleocapsid, RBD, P.1, B.1.617.1, Gamma, D614G, Alpha, plasma, Beta, B.1.1.529, assessment, BBIBP-CorV, N-terminal domain, Human angiotensin-converting enzyme 2, Sinopharm, angiotensin, Injury, hyperimmune globulin, weight loss, Globulin, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, assessments, healthy donors, global public health, IFNAR, viral loads, human Angiotensin-converting enzyme, neutralization potency, RBD binding, neutralization titer, pseudotyped, high-affinity binding, block, Effect, effective, hyperimmune, healthy donor, significantly, subsequent, reduced, variety, the receptor-binding domain, the S protein, reduction in, exhibit, 【제목키워드】 COVID-19, potent,