Abstract
The spread of the corona virus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been intensifying in the past year, posing a huge threat to global health. There is an urgent need for effective drugs and vaccines to fight the COVID-19, but their advent may not be quite fast. Drug repurposing is a feasible strategy in the current situation, which could greatly shorten drug development time and help to response quickly to the novel virus outbreak. It has been reported that histamine H 1 receptor antagonists have broad-spectrum antiviral effects. Therefore, in this study, we aim to screen potential drugs among histamine H 1 receptor antagonists that may inhibit SARS-CoV-2 infection. Based on the model of angiotensin-converting enzyme 2 (ACE2) overexpressing HEK293T cell membrane chromatography (CMC), five FDA-approved histamine H 1 receptor antagonists were found to have bioaffinity to ACE2. Then we determined the interaction between these drugs and ACE2 by frontal analysis and surface plasmon resonance (SPR), which consistently demonstrated that these hits bind to ACE2 at micromolar levels of affinity. Through the pseudovirus assay, we finally identified that doxepin could inhibit SARS-CoV-2 spike pseudovirus from entering the ACE2-expressing cell, reducing the infection rate to 25.82%. These preliminary results indicate that the histamine H 1 receptor antagonist, doxepin, is a viable drug candidate for clinical trials. Therefore, we hope the work timely provides rational help for developing anti-SARS-CoV-2 drugs to control the rapid spread of SARS-CoV-2.
Keywords: Angiotensin-converting enzyme 2; Doxepin; Drug repositioning; Histamine H(1) receptor antagonist; SARS-CoV-2.
【저자키워드】 SARS-CoV-2, drug repositioning, angiotensin-converting enzyme 2, Doxepin, Histamine H(1) receptor antagonist, 【초록키워드】 COVID-19, Drug repurposing, SARS-CoV-2, ACE2, Vaccine, coronavirus, pandemic, SARS-COV-2 infection, Infection, clinical trials, antiviral effects, drug, surface plasmon resonance, severe acute respiratory syndrome Coronavirus, virus, SPR, anti-SARS-CoV-2, angiotensin-converting enzyme 2, global health, effective drugs, Spread, Health, pseudovirus, outbreak, membrane, receptor, infection rate, affinity, Angiotensin-converting enzyme, Interaction, Doxepin, Analysis, angiotensin, Histamine, Virus Disease, receptor antagonist, acute respiratory syndrome, surface plasmon, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, enzyme, help, drug candidate, antagonist, FIVE, Cell, caused, reported, provide, reducing, demonstrated, feasible, CMC, effective drug, HEK293T cell, inhibit SARS-CoV-2, overexpressing, 【제목키워드】 SARS-CoV-2, ACE2, spike, pseudovirus, repositioning, receptor, Doxepin, inhibit,