Abstract
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca 2+ from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a “clickable” NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca 2+ signaling. JPT2 was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal system. Thus, JPT2 is a component of the NAADP receptor complex that is essential for TPC-dependent Ca 2+ signaling and control of coronaviral entry.
【초록키워드】 SARS-CoV-2, Release, coronavirus, severe acute respiratory syndrome Coronavirus, Protein, sensitivity, pseudovirus, Signaling, regulate, adenine, phosphate, second messenger, identity, acute respiratory syndrome, Activation, nicotinic acid, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, endolysosomal system, component, Coronaviral, homolog, translocation, acidic organelles, receptor complex, coronaviral entry, JPT2, required, events, acidic organelle, endolysosomal, Jupiter, 【제목키워드】 Signaling, Essential,