Abstract
Research conducted on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogenesis and coronavirus disease 2019 (COVID-19) generally focuses on the systemic host response, especially that generated by severely ill patients, with few studies investigating the impact of acute SARS-CoV-2 at the site of infection. We show that the nasal microbiome of SARS-CoV-2-positive patients (CoV + , n = 68) at the time of diagnosis is unique when compared to CoV – healthcare workers (n = 45) and CoV – outpatients (n = 21). This shift is marked by an increased abundance of bacterial pathogens, including Pseudomonas aeruginosa, which is also positively associated with viral RNA load. Additionally, we observe a robust host transcriptional response in the nasal epithelia of CoV + patients, indicative of an antiviral innate immune response and neuronal damage. These data suggest that the inflammatory response caused by SARS-CoV-2 infection is associated with an increased abundance of bacterial pathogens in the nasal cavity that could contribute to increased incidence of secondary bacterial infections.
Keywords: COVID-19; Pseudomonas aeruginosa; RNA-seq; SARS-CoV-2; coinfection; inflammation; nasal microbiome; viral RNA load.
【저자키워드】 COVID-19, SARS-CoV-2, Inflammation, Coinfection, RNA-Seq, Pseudomonas aeruginosa, nasal microbiome, viral RNA load., 【초록키워드】 coronavirus disease, Coronavirus disease 2019, coronavirus, Pathogenesis, innate immune response, Antiviral, SARS-COV-2 infection, Infection, Diagnosis, nasal, host response, severe acute respiratory syndrome Coronavirus, healthcare worker, Coinfection, RNA-Seq, Microbiome, CoV, Pathogens, Secondary bacterial infections, Bacterial pathogens, incidence, Host transcriptional response, Nasal cavity, viral RNA load, Bacterial infections, patients, Outpatient, Inflammatory response, Pseudomonas aeruginosa, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, These data, SARS-CoV-2-positive patients, severely ill patients, bacterial pathogen, epithelia, robust, observé, caused, conducted, contribute, unique, neuronal, Pseudomona, SARS-CoV-2-positive patient, 【제목키워드】 Pseudomonas aeruginosa,