Abstract
The best and most effective way to combat pandemics is to use effective vaccines and live attenuated vaccines are among the most effective vaccines. However, one of the major problems is the length of time it takes to get the attenuated vaccines. Today, the CRISPR toolkit (Clustered Regularly Inerspaced Short Palindromic Repeats) has made it possible to make changes with high efficiency and speed. Using this toolkit to make point mutations on the RNA virus’s genome in a coculture of permissive and nonpermissive cells and under controlled conditions can accelerate changes in the genome and accelerate natural selection to obtain live attenuated vaccines.
Keywords: Attenuated vaccines; CRISPR toolkit; Natural selection; Point mutation; RNA viruses.
【저자키워드】 RNA viruses, natural selection, Attenuated vaccines, CRISPR toolkit, Point mutation, 【초록키워드】 Genome, CRISPR, RNA viruses, Pandemics, natural selection, point mutations, Live attenuated vaccines, RNA virus, speed, change, Attenuated vaccines, Point mutation, Live attenuated vaccine, Efficiency, natural, Effective vaccines, problem, toolkit, effective, Cell, coculture, condition, changes in, effective vaccine, accelerate, Palindromic, Point, 【제목키워드】 SARS-CoV-2, RNA, CRISPR, Selection, natural, enhanced, Rate, Produce,