Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a major threat to human health. As a unique putative protein of SARS-CoV-2, the N-terminus of ORF10 can be recognized by ZYG11B, a substrate receptor of the Cullin 2-RING E3 ubiquitin ligase (CRL2). Here we elucidated recognition mechanism of ORF10 N-terminus by ZYG11B through presenting the crystal structure of ZYG11B bound to ORF10 N-terminal peptide. Our work expands the current understanding of ORF10 interaction with ZYG11B, and may also inspire the development of novel therapies for COVID-19.
Keywords: Crystal structure; Cullin-RING E3 ligase; ORF10; ZYG11B.
All Keywords
【저자키워드】 crystal structure, ORF10, Cullin-RING E3 ligase, ZYG11B., 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Coronavirus disease 2019, coronavirus, therapy, peptide, severe acute respiratory syndrome Coronavirus, Protein, Health, receptor, crystal structure, mechanism, ORF10, Interaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, crystal, E3 ubiquitin ligase, N-terminal, N-terminus, ZYG11B, caused, unique, presenting, expand, Cullin,
【저자키워드】 crystal structure, ORF10, Cullin-RING E3 ligase, ZYG11B., 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Coronavirus disease 2019, coronavirus, therapy, peptide, severe acute respiratory syndrome Coronavirus, Protein, Health, receptor, crystal structure, mechanism, ORF10, Interaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, crystal, E3 ubiquitin ligase, N-terminal, N-terminus, ZYG11B, caused, unique, presenting, expand, Cullin,
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중증급성호흡기증후군 코로나바이러스 2(SARS-CoV-2)로 인한 코로나바이러스 질병 2019(COVID-19)가 인간 건강에 대한 주요 위협이 되었습니다. SARS-CoV-2의 독특한 추정 단백질로서 ORF10의 N-말단은 Cullin 2-RING E3 ubiquitin ligase(CRL2)의 기질 수용체인 ZYG11B에 의해 인식될 수 있습니다. 여기에서 우리는 ORF10 N-말단 펩타이드에 결합된 ZYG11B의 결정 구조를 제시함으로써 ZYG11B에 의한 ORF10 N-말단의 인식 메커니즘을 설명하였다. 우리의 작업은 ORF10과 ZYG11B의 상호 작용에 대한 현재의 이해를 확장하고 COVID-19에 대한 새로운 치료법 개발에 영감을 줄 수도 있습니다.
{{ 키워드: }} 결정 구조; Cullin-RING E3 리가제; ORF10; ZYG11B.