Abstract
Although tremendous effort has been exerted to elucidate the pathogenesis of severe COVID-19 cases, the detailed mechanism of moderate cases, which accounts for 90% of all patients, remains unclear yet, partly limited by lacking the biopsy tissues. Here, we established the COVID-19 infection model in cynomolgus macaques (CMs), monitored the clinical and pathological features, and analyzed underlying pathogenic mechanisms at early infection stage by performing proteomic and metabolomic profiling of lung tissues and sera samples from COVID-19 CMs models. Our data demonstrated that innate immune response, neutrophile and platelet activation were mainly dysregulated in COVID-19 CMs. The symptom of neutrophilia, lymphopenia and massive “cytokines storm”, main features of severe COVID-19 patients, were greatly weakened in most of the challenged CMs, which are more semblable as moderate patients. Thus, COVID-19 model in CMs is rational to understand the pathogenesis of moderate COVID-19 and may be a candidate model to assess the safety and efficacy of therapeutics and vaccines against SARS-CoV-2 infection.
Keywords: SARS-CoV-2 infection; cynomolgus macaque; immune response; metabolomics; proteomics; the early stage.
【저자키워드】 metabolomics, proteomics, immune response, SARS-COV-2 infection, cynomolgus macaque, the early stage., 【초록키워드】 COVID-19, SARS-CoV-2, metabolomics, proteomics, Efficacy, platelet activation, Vaccine, immune response, Cytokines, Pathogenesis, innate immune response, severe COVID-19, Therapeutics, SARS-COV-2 infection, Symptom, immune, Moderate COVID-19, lymphopenia, Features, COVID-19 infection, sera, metabolomic profiling, pathogenic mechanism, moderate, early stage, patients, mechanism, proteomic, cynomolgus macaques, early infection, tissues, severe COVID-19 patients, effort, lung tissue, neutrophilia, lung tissues, feature, weakened, analyzed, demonstrated, dysregulated, 【제목키워드】 macaque, characterization, Stage,