Abstract
(Mi)RNAs are important biomarkers for cancers diagnosis and pandemic diseases, which require fast, ultrasensitive, and economical detection strategies to quantitatively detect exact (mi)RNAs expression levels. The novel coronavirus disease (SARS-CoV-2) has been breaking out globally, and RNA detection is the most effective way to identify the SARS-CoV-2 virus. Here, we developed an ultrasensitive poly-l-lysine (PLL)-functionalized graphene field-effect transistor (PGFET) biosensor for breast cancer miRNAs and viral RNA detection. PLL is functionalized on the channel surface of GFET to immobilize DNA probes by the electrostatic force. The results show that PGFET biosensors can achieve a (mi)RNA detection range of five orders with a detection limit of 1 fM and an entire detection time within 20 min using 2 μL of human serum and throat swab samples, which exhibits more than 113% enhancement in terms of sensitivity compared to that of GFET biosensors. The performance enhancement mechanisms of PGFET biosensors were comprehensively studied based on an electrical biosensor theoretical model and experimental results. In addition, the PGFET biosensor was applied for the breast cancer miRNA detection in actual serum samples and SARS-CoV-2 RNA detection in throat swab samples, providing a promising approach for rapid cancer diagnosis and virus screening.
【초록키워드】 coronavirus disease, SARS-CoV-2, Breast cancer, pandemic, Biomarker, Diseases, Cancer, miRNA, Diagnosis, SARS-CoV-2 virus, novel coronavirus disease, virus, Novel coronavirus, RNA, DNA, sensitivity, miRNAs, cancers, field-effect transistor, Viral RNA, human serum, mechanism, Graphene, Detection limit, SARS-CoV-2 RNA detection, expression levels, experimental results, throat swab, serum sample, cancer diagnosis, viral RNA detection, probe, approach, FIVE, effective, poly-l-lysine, identify, detect, addition, applied, electrical, electrostatic, exhibit, the SARS-CoV-2 virus, 【제목키워드】 miRNA, detection, biosensor, Breast, SARS-CoV-2 RNA, transistor,