Abstract
Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (Cu x O/TiO 2 ) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the Cu x O/TiO 2 photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the Cu x O/TiO 2 photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The Cu x O/TiO 2 photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night.
【초록키워드】 COVID-19, SARS-CoV-2, coronavirus, Antiviral, sodium, SARS-CoV-2 virus, severe acute respiratory syndrome Coronavirus, delta variant, virus, RNAs, ELISA, Spike protein, RNA, Spread, polymerase chain reaction, enzyme-linked immunosorbent assay, RT-qPCR, inactivation, titanium, reverse transcription, copper, disease, Quantitative, mechanism, inactivated, titanium dioxide, pandemic coronavirus, Spike proteins, mutant strain, Chain Reaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, enzyme, polyacrylamide, fluorescent, illumination, quantitative polymerase chain reaction, denaturation, fragmentation, gel electrophoresis, Night, infectious risk, photocatalyst, effective, enzyme-linked immunosorbent, SDS-PAGE, polymerase chain, copper oxide, PROTECT, examined, addition, investigated, reduce, damaged, the SARS-CoV-2 virus, 【제목키워드】 SARS-CoV-2, variant,