Abstract
In this protocol, we describe global proteome profiling for the respiratory specimen of COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. In this protocol, details for identifying host, viral, or bacterial proteome (Meta-proteome) are provided. Major steps of the protocol include virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry (HRMS)-based analysis, and downstream bioinformatics analysis. For complete details on the use and execution of this profile, please refer to Maras et al. (2021).
Keywords: Health Sciences; Mass Spectrometry; Proteomics.
All Keywords
【저자키워드】 proteomics, mass spectrometry, Health Sciences, 【초록키워드】 proteomics, mass spectrometry, protocol, virus inactivation, Protein, Patient, peptides, H1N1, proteome, quantification, Bioinformatics analysis, COVID-19 patients, Bacterial, Analysis, High-resolution, digestion, Major, mass, specimen, downstream, Host, Complete, H1N1 patients, include, provided, with COVID-19, 【제목키워드】 VTM, proteome, Analysis, COVID-19 patient, specimen,
【저자키워드】 proteomics, mass spectrometry, Health Sciences, 【초록키워드】 proteomics, mass spectrometry, protocol, virus inactivation, Protein, Patient, peptides, H1N1, proteome, quantification, Bioinformatics analysis, COVID-19 patients, Bacterial, Analysis, High-resolution, digestion, Major, mass, specimen, downstream, Host, Complete, H1N1 patients, include, provided, with COVID-19, 【제목키워드】 VTM, proteome, Analysis, COVID-19 patient, specimen,
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이 프로토콜에서는 COVID-19 환자, COVID-19가 의심되는 환자 및 H1N1 환자의 호흡기 표본에 대한 글로벌 프로테옴 프로파일링을 설명합니다. 이 프로토콜에서는 호스트, 바이러스 또는 박테리아 프로테옴(메타 프로테옴)을 식별하기 위한 세부 정보가 제공됩니다. 프로토콜의 주요 단계에는 바이러스 비활성화, 단백질 정량화 및 소화, 펩타이드의 탈염, 고해상도 질량 분석(HRMS) 기반 분석 및 다운스트림 생물정보학 분석이 포함됩니다. 이 프로필의 사용 및 실행에 대한 자세한 내용은 Maras et al. (2021).
{{ 키워드: }} 건강 과학; 질량 분석; 단백질체학.