Abstract
Metagenomic DNA sequencing is a powerful tool to characterize microbial communities but is sensitive to environmental DNA contamination, in particular when applied to samples with low microbial biomass. Here, we present Sample-Intrinsic microbial DNA Found by Tagging and sequencing (SIFT-seq) a metagenomic sequencing assay that is robust against environmental DNA contamination introduced during sample preparation. The core idea of SIFT-seq is to tag the DNA in the sample prior to DNA isolation and library preparation with a label that can be recorded by DNA sequencing. Any contaminating DNA that is introduced in the sample after tagging can then be bioinformatically identified and removed. We applied SIFT-seq to screen for infections from microorganisms with low burden in blood and urine, to identify COVID-19 co-infection, to characterize the urinary microbiome, and to identify microbial DNA signatures of sepsis and inflammatory bowel disease in blood.
【초록키워드】 COVID-19, Sequencing, Infection, Sepsis, Inflammatory bowel disease, DNA, Contamination, Co-infection, Microbiome, Isolation, Urine, microorganisms, DNA sequencing, microbial community, metagenomic sequencing, Blood, microorganism, microbial, lAbel, powerful tool, Microbial communities, SIFT, robust, identify, applied, introduced, recorded, Tagging, 【제목키워드】 DNA, Contamination, robust, metagenomic,