Abstract
In the Fall of 2020, university campuses in the United States resumed on-campus instruction and implemented wastewater monitoring for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While quantitative polymerase chain reaction (qPCR) tests were deployed successfully to detect viral RNA in wastewater across campuses, the feasibility of detecting viral variants from a residential building like a dormitory was unclear. Here, we demonstrate that wastewater surveillance from a dormitory with at least three infected students could lead to the identification of viral genomes with more than 95% coverage. Our results indicate that viral variant detection from wastewater is achievable at a dormitory and that coronavirus disease 2019 (COVID-19) wastewater surveillance programs will benefit from the implementation of viral whole genome sequencing at universities.
Keywords: COVID-19; Mutation; SARS-CoV-2; Sewage; Variant; Virus; Wastewater.
【저자키워드】 COVID-19, SARS-CoV-2, Mutation, variant, virus, Wastewater., sewage, 【초록키워드】 coronavirus disease, Coronavirus disease 2019, coronavirus, Mutation, feasibility, Sequencing, variant, severe acute respiratory syndrome Coronavirus, polymerase chain reaction, wastewater, Whole genome sequencing, Coverage, Surveillance, qPCR, implementation, Viral variants, viral genomes, Viral RNA, sewage, United States, viral variant, Quantitative, Dormitory, viral genome, universities, Chain Reaction, residential building, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, quantitative polymerase chain reaction, whole genome, while, benefit, polymerase chain, detect, the United State, 【제목키워드】 SARS-CoV-2 variant, genomic,