Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection often causes severe complications and even death. However, asymptomatic infection has also been reported, highlighting the difference in immune responses among individuals. Here we performed single-cell chromatin accessibility and T cell-receptor analyses of peripheral blood mononuclear cells collected from individuals convalescing from COVID-19 and healthy donors. Chromatin remodelling was observed in both innate and adaptive immune cells in the individuals convalescing from COVID-19. Compared with healthy donors, recovered individuals contained abundant TBET-enriched CD16 + and IRF1-enriched CD14 + monocytes with sequential trained and activated epigenomic states. The B-cell lineage in recovered individuals exhibited an accelerated developmental programme from immature B cells to antibody-producing plasma cells. Finally, an integrated analysis of single-cell T cell-receptor clonality with the chromatin accessibility landscape revealed the expansion of putative SARS-CoV-2-specific CD8 + T cells with epigenomic profiles that promote the differentiation of effector or memory cells. Overall, our data suggest that immune cells of individuals convalescing from COVID-19 exhibit global remodelling of the chromatin accessibility landscape, indicative of the establishment of immunological memory.
【초록키워드】 COVID-19, severe acute respiratory syndrome coronavirus 2, Monocytes, SARS-CoV-2, coronavirus, immune response, adaptive, antibody, T cells, Infection, severe acute respiratory syndrome Coronavirus, CD8, Immunological memory, coronavirus 2, monocyte, Peripheral blood, B cell, Severe acute respiratory syndrome, T cell, immune responses, asymptomatic infection, Lineage, Peripheral blood mononuclear cells, death, immune cells, expansion, differentiation, IRF1, single-cell, B-cell, Plasma cells, CD16, Analysis, Immune cell, Memory cells, mononuclear cell, acute respiratory syndrome, acute respiratory syndrome coronavirus, healthy donors, profile, individual, CD14, severe complications, chromatin, performed, collected, reported, exhibited, activated, promote, cause, individuals, immature, accelerated, developmental, highlighting, severe complication, epigenomic, 【제목키워드】 COVID-19, Immunity, Immune cell, individual, reveal, epigenomic,