Abstract
An essential step in SARS-CoV-2 infection is binding the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to the ACE2 receptor on the surface of host cells. Therefore, variation in this region can have crucial effects on clinical outcomes and the emergence of variants of concern (VOCs) and variants of interest (VOIs). In this cross-sectional descriptive study, 54 patients with SARS-COV-2 infection were enrolled. After collecting samples and identifying the virus using the One-Step Real-Time qRT-PCR technique and confirming the viral infection, the region containing the RBD region for detection of any mutations was amplified using the Nested-PCR method. Finally, to identify probable mutations, the Nested-PCR product was sequenced. Our data show that the most mutant strains in circulation in our population are the delta variant (90.74%), alpha variant (5.56%), and omicron variant (3.70%), respectively. Pangolin Lineages strains were B.1.1.7(Alpha variant), B.1.617.2(Delta variant) and B.1.1.529(Omicron variant). Also, the mutation profile of variants suggests that N501Y, T478K, and D614G amino acid substitutions, are the significant mutations in the alpha and delta variants that are common with the Omicron variant. The highest frequency of clinical signs in the patients were: lung involvement (42.59%); fever, chills (40.74%); body pain (15%), and other signs (1.67%). Our data revealed that SARS-COV-2 RBD region variation results in substituting essential amino acids and the emergence of the new variant. We can consider it as a predictor for monitoring the emergence of variants of concerns and viral outcomes.
Keywords: Mutations; RBD; SARS-CoV2; SPIKE; Variant.
【저자키워드】 SARS-CoV2, spike, mutations, RBD, Variant., 【초록키워드】 viral infection, Mutation, cross-sectional, SARS-COV-2 infection, lung involvement, qRT-PCR, Variation, variant, Infection, variants of concern, ACE2 receptor, omicron, delta variant, virus, variants, clinical outcomes, Spike protein, Clinical outcome, outcomes, Receptor-binding domain, B.1.1.7, N501Y, SARS-CoV-2 spike protein, Fever, VOCs, Patient, Pain, amino acids, D614G, Alpha, variants of interest, Omicron variant, Clinical signs, B.1.1.529, Alpha variant, circulation, VOIs, Amino acid, Frequency, pangolin, mutant strain, strain, amino acid substitutions, host cells, T478K, body pain, domain, alpha and delta variants, chills, RBD region, chill, Clinical sign, Effect, essential amino acids, enrolled, highest, amplified, identify, sequenced, the patient, the RBD, binding the receptor-binding, the SARS-CoV-2, 【제목키워드】 Mutation, binding domain,