Abstract
The RNA dependent RNA polymerase (RdRp) plays crucial role in virus life cycle by replicating the viral genome. The SARS-CoV-2 is an RNA virus that rapidly spread worldwide and acquired mutations. This study was carried out to identify mutations in RdRp as the SARS-CoV-2 spread in India. We compared 50217 RdRp sequences reported from India with the first reported RdRp sequence from Wuhan, China to identify 223 mutations acquired among Indian isolates. Our protein modelling study revealed that several mutants can potentially alter stability and flexibility of RdRp. We predicted the potential B cell epitopes contributed by RdRp and identified thirty-six linear continuous and twenty-five discontinuous epitopes. Among 223 RdRp mutants, 44% of them localises in the B cell epitopes region. Altogether, this study highlights the need to identify and characterize the variations in RdRp to understand the impact of these mutations on SARS-CoV-2.
Keywords: B cell epitopes; COVID-19; India; Mutations; RNA dependent RNA polymerase (RdRp); SARS-CoV-2.
【저자키워드】 COVID-19, India, mutations, SARS-CoV-2., B cell epitopes, RNA dependent RNA polymerase (RdRp), 【초록키워드】 SARS-CoV-2, Mutation, Variation, India, RNA dependent RNA polymerase, RNA, B cell, Spread, Protein, Epitopes, stability, Wuhan, RdRP, mutants, variations, mutant, RNA polymerase, RNA virus, flexibility, B cell epitopes, viral genome, B cell epitope, isolates, life cycle, Virus life cycle, RdRp sequences, sequence, Alter, highlight, Wuhan, China, predicted, identify, carried, reported, linear, contributed, RdRp sequence, the SARS-CoV-2, 【제목키워드】 genetic diversity, B-cell epitope, SARS-CoV-2 RNA, RNA polymerase, Alter,