Abstract
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), an enveloped RNA virus transmits by droplet infection thus affects the respiratory system. Different genomes have been reported globally for SARS-CoV-2 with moderate level of mutation which makes it harder to combat the virus. Mutational profiling and the relevant evolutionary aspect of coronavirus proteins namely spike glycoprotein, membrane protein, envelope protein, nucleoprotein, ORF1ab, ORF3a, ORF6, ORF7a, ORF7b and ORF8 were studied by in silico experiments. Clustering of the protein sequences and calculation of residue relative abundance were done to get an idea about the protein conservancy as well as finding out some representative sequences for phylogenetic and ancestral reconstruction. By mutational profiling and mutation analysis, the effect of mutations on the protein stability and their functional implication were studied. This study indicates the mutational effect on the proteins and their relevance in evolution, which directs us towards a better understanding of these variations and diversification of SARS-CoV-2 for useful future therapeutic study and thus aid in designing therapeutic agents keeping the highly variable regions in mind.
Keywords: Clustering; Mutation profiling; Mutation stability; SARS-CoV-2 proteins; Shannon entropy.
【저자키워드】 Clustering, Mutation profiling, Mutation stability, SARS-CoV-2 proteins, Shannon entropy, 【초록키워드】 Evolution, SARS-CoV-2, Mutation, Variation, Genome, spike glycoprotein, Infection, severe acute respiratory syndrome coronavirus-2, in silico, severe acute respiratory syndrome Coronavirus, virus, Severe acute respiratory syndrome, Protein, Region, ORF3a, ORF8, membrane protein, envelope protein, therapeutic agent, therapeutic agents, ORF6, Respiratory system, glycoprotein, protein stability, nucleoprotein, RNA virus, moderate, ORF7a, ORF7b, Coronavirus-2, Analysis, SARS-CoV-2 proteins, ORF1ab, Phylogenetic, acute respiratory syndrome, acute respiratory syndrome coronavirus, residue, sequence, idea, calculation, mind, protein sequences, protein sequence, Affect, Droplet infection, Shannon entropy, coronavirus protein, reported, functional, indicate, experiments, future therapeutic, 【제목키워드】 SARS-CoV-2,