Abstract
COVID-19 is one of the fatal pandemic throughout the world. For cellular fusion, its antigenic peptides are presented by major histocompatibility complex (MHC) in humans. Therefore, exploration into residual interaction details of CoV2 with MHCs shall be a promising point for instigating the vaccine development. Envelope (E) protein, the smallest outer surface protein from SARS-CoV2 genome was found to possess the highest antigenicity and is therefore used to identify B-cell and T-cell epitopes. Four novel mutations (T55S, V56F, E69R and G70del) were observed in E-protein of SARS-CoV2 after evolutionary analysis. It showed a coil➔helix transition in the protein conformation. Antigenic variability of the epitopes was also checked to explore the novel mutations in the epitope region. It was found that the interactions were more when SARS-CoV2 E-protein interacted with MHC-I than with MHC-II through several ionic and H-bonds. Tyr42 and Tyr57 played a predominant role upon interaction with MHC-I. The higher ΔG values with lesser dissociation constant values also affirm the stronger and spontaneous interaction by SARS-CoV2 proteins with MHCs. On comparison with the consensus E-protein, SARS-CoV2 E-protein showed stronger interaction with the MHCs with lesser solvent accessibility. E-protein can therefore be targeted as a potential vaccine target against SARS-CoV2.
Keywords: COVID-19; Epitope identification; Interactions with MHCs.
【저자키워드】 COVID-19, epitope identification, Interactions with MHCs., 【초록키워드】 Vaccine, Vaccine development, SARS-CoV2, pandemic, Mutation, Protein, Epitopes, humans, peptides, envelope, antigenicity, T-cell epitopes, epitope, MHC, Dissociation constant, B-cell, Protein conformation, cellular, Interaction, Analysis, major histocompatibility complex, MHC-I, Consensus, Variability, H-bonds, solvent, exploration, surface protein, MHC-II, B-cell and T-cell epitopes, MHCs, SARS-CoV2 genome, highest, identify, the vaccine, predominant, histocompatibility complex, checked, antigenic peptide, 【제목키워드】 Vaccine, SARS-CoV2, docking, molecular, development,