Immature immune system and immune tolerance induced by exposure to HBeAg in utero and/or shortly after infection in newborns were reportedly the causes of chronic HBV infection. To investigate the effect of maternal-derived HBeAg on neonatal T cell immunity, we analysed and compared T cell phenotypes and functions among neonates born to HBsAg^{+} /HBeAg^{+} mothers (HBeAg^{+} neonates), HBsAg^{+} /HBeAg^{-} mothers (HBeAg^{-} neonates) and healthy control mothers (HC neonates), using flow cytometry. The results showed that neonatal T cell phenotypes were similar regardless of HBeAg exposure. Upon anti-CD3 and anti-CD28 stimulation in HBeAg^{+} neonates, CD4^{+} T cell production of IFN-γ (P < .05) was significantly enhanced, while CD8^{+} T cells secreted significantly more IL-2 compared with those in HBeAg^{-} and HC groups (P < .05). Moreover, similar levels of IFN-γ and IL-10 were observed in the culture supernatant after stimulation with rHBsAg, rHBcAg or rHBeAg among HBeAg^{+} , HBeAg^{-} and HC neonates, whereas HBeAg^{+} neonates produced more TNF-α than HBeAg^{-} neonates upon stimulation with rHBcAg. In conclusion, the results indicated that the HBsAg^{+} /HBeAg^{+} maternal environment did not influence the phenotypes of cord blood T cells but boosted neonatal non-specific Th1-type cytokine production.
【저자키워드】 T cells, Neonates, HBeAg,