Abstract
The global pandemic of coronavirus disease 2019 (COVID-19) has significant impact on the entire human society. However, in the face of continually emerging more contagious SARS-CoV-2 variant, the risk to bog down into more severe crisis is around us anytime. Here, we introduce an isothermal, ultrasensitive method for identifying important SNV mutations of SARS-CoV-2. It is based on combined specificity of toehold-assisted linear probe ligation and in vitro transcription signal enlargement, TLT. A ready-to-use panel of TLT assay is developed including detection of 80 crucial SARS-CoV-2 SNVs, by which people could response to the next coming contagious virus variant more rapidly. These advanced point-of-care features make TLT one good approach for large scale population testing of special SARS-CoV-2 variants of interesting.
Keywords: COVID-19; Ligase chain reaction; SARS-CoV-2; Toehold-mediated strand displacement reaction.
【저자키워드】 COVID-19, SARS-CoV-2, Ligase chain reaction, Toehold-mediated strand displacement reaction., 【초록키워드】 coronavirus disease, Coronavirus disease 2019, Mutation, variant, SARS-CoV-2 variant, risk, virus, global pandemic, point-of-care, specificity, SNV, isothermal, Chain Reaction, contagious, SNVs, probe, Ligase, approach, feature, linear, in vitro transcription, 【제목키워드】 Transcription, genotyping,