Human leukocyte antigen (HLA) is a group of molecules involved in inflammatory and infective responses. We evaluated blood sHLA-E and sHLA-G levels in hospitalized COVID-19 patients with respiratory failure and their relationship with clinical evolution, changes in endothelial activation biomarker profile, and neutrophil adhesion. sHLA-E, sHLA-G, and endothelial activation biomarkers were quantified by ELISA assay in plasma samples. Neutrophil adhesion to endothelium was assessed in the presence/absence of patients’ plasma samples. At admission, plasma levels of sHLA-G and sHLA-E were significantly higher in COVID-19 patients with respiratory failure compared to controls. COVID-19 clinical improvement was associated with increased sHLA-G plasma levels. In COVID-19, but not in control patients, an inverse correlation was found between serum sICAM-1 and E-selectin levels and plasma sHLA-G values. The in vitro analysis of activated endothelial cells confirmed the ability of HLA-G molecules to control sICAM-1 and sE-selectin expression via CD160 interaction and FGF2 induction and consequently neutrophil adhesion. We suggest a potential role for sHLA-G in improving COVID-19 patients’ clinical condition related to the control of neutrophil adhesion to activated endothelium.
【저자키워드】 COVID-19, neutrophil, HLA-G, E-selectin, ICAM-1, CD160, 【초록키워드】 Biomarker, Respiratory failure, neutrophil, human leukocyte antigen, HLA-G, CD160, in vitro, Endothelium, serum, endothelial cells, Clinical improvement, plasma, HLA, molecules, expression, Admission, COVID-19 patients, Blood, ELISA assay, hospitalized COVID-19 patient, Endothelial cell, Interaction, Analysis, Inflammatory, Plasma levels, COVID-19 patient, leukocyte, endothelial, Activation, hospitalized COVID-19 patients, plasma samples, control patients, clinical condition, clinical evolution, FGF2, inverse correlation, selectin, infective, responses, controls, involved, evaluated, activated, changes in, significantly higher, quantified, 【제목키워드】 neutrophil, adhesion, Improved, Increased,