Abstract
We developed a next-generation SARS-CoV-2 sequencing platform and obtained the first SARS-CoV-2 sequences from patients in Croatia at the beginning of the COVID-19 outbreak in the spring of 2020. Integrating the sequencing and the epidemiological data, we show that patients were infected with different SARS-CoV-2 variants belonging to different clades (mostly G and GH). This result confirms that there was widespread virus transmission early in 2020. Interestingly, we identified a unique mutation resulting in a V13I substitution in Nsp5A, the main viral protease, in a patient who had not received antiviral therapy.
All Keywords
【초록키워드】 antiviral therapy, Mutation, Sequencing, SARS-CoV-2 variant, virus transmission, protease, COVID-19 outbreak, SARS-CoV-2 variants, Croatia, Patient, clade, epidemiological data, SARS-CoV-2 sequencing, platform, SARS-CoV-2 sequences, spring, SARS-CoV-2 sequence, widespread, resulting, were infected, unique, 【제목키워드】 SARS-CoV-2 variant, initial,
【초록키워드】 antiviral therapy, Mutation, Sequencing, SARS-CoV-2 variant, virus transmission, protease, COVID-19 outbreak, SARS-CoV-2 variants, Croatia, Patient, clade, epidemiological data, SARS-CoV-2 sequencing, platform, SARS-CoV-2 sequences, spring, SARS-CoV-2 sequence, widespread, resulting, were infected, unique, 【제목키워드】 SARS-CoV-2 variant, initial,
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우리는 차세대 SARS-CoV-2 시퀀싱 플랫폼을 개발하고 2020년 봄 COVID-19 발병 초기에 크로아티아 환자로부터 최초의 SARS-CoV-2 시퀀스를 획득했습니다. 시퀀싱과 역학 데이터를 통합하여, 우리는 환자들이 서로 다른 계통군(주로 G 및 GH)에 속하는 서로 다른 SARS-CoV-2 변이체에 감염되었음을 보여줍니다. 이 결과는 2020년 초에 광범위한 바이러스 전파가 있었음을 확인시켜줍니다. 흥미롭게도 우리는 항바이러스 치료를 받지 않은 환자에서 주요 바이러스 프로테아제인 Nsp5A에서 V13I 치환을 초래하는 고유한 돌연변이를 확인했습니다.