Abstract
The diagnosis of COVID-19 is based on detection of SARS-CoV-2 in oro-/nasopharyngel swabs, but due to discomfort and minor risk during the swab procedure, detection of SARS-CoV-2 has been investigated in other biological matrixes. In this proof-of-concept study, individuals with confirmed SARS-CoV-2 infection performed a daily air sample for five days. Air samples were obtained through a non-invasive electrostatic air sampler. Detection of SARS-CoV-2 RNA was determined with qRT-PCR. The association of positive samples with different exposures was evaluated through mixed-effect models. We obtained 665 air samples from 111 included participants with confirmed SARS-CoV-2 infection. Overall, 52 individuals (46.8%) had at least one positive air sample, and 129 (19.4%) air samples were positive for SARS-CoV-2. Participants with symptoms or a symptom duration ≤ four days had significantly higher odds of having a positive air sample. Cycle threshold values were significantly lower in samples obtained ≤ 4 days from symptom onset. Neither variant of SARS-CoV-2 nor method of air sampling were associated with a positive air sample. We demonstrate that SARS-CoV-2 is detectable in human breath by electrostatic air sampling with the highest detection rate closest to symptom onset. We suggest further evaluation of the air sampling technique to increase sensitivity.
【초록키워드】 SARS-CoV-2, SARS-COV-2 infection, qRT-PCR, risk, detection, Symptom, sensitivity, symptom duration, swabs, breath, SARS-CoV-2 RNA, Swab, detection rate, association, Cycle threshold values, symptom onset, Non-invasive, cycle, individual, discomfort, participant, threshold value, positive, significantly lower, positive sample, FIVE, variant of SARS-CoV-2, highest, performed, investigated, detectable, evaluated, was determined, significantly higher, electrostatic, diagnosis of COVID-19, 【제목키워드】 SARS-CoV-2, detection, breath, individual,