Abstract
Although there are several reports in the literature of SARS-CoV-2 infection in cats, few SARS-CoV-2 sequences from infected cats have been published. In this study, SARS-CoV-2 infection was evaluated in two cats by clinical observation, molecular biology (qPCR and NGS), and serology (microsphere immunoassay and seroneutralization). Following the observation of symptomatic SARS-CoV-2 infection in two cats, infection status was confirmed by RT-qPCR and, in one cat, serological analysis for antibodies against N-protein and S-protein, as well as neutralizing antibodies. Comparative analysis of five SARS-CoV-2 sequence fragments obtained from one of the cats showed that this infection was not with one of the three recently emerged variants of SARS-CoV-2. This study provides additional information on the clinical, molecular, and serological aspects of SARS-CoV-2 infection in cats.
Keywords: NGS; One Health; SARS-CoV-2; cats; mild-respiratory clinical signs; serology.
【저자키워드】 SARS-CoV-2, serology, NGS, cats, one health, mild-respiratory clinical signs, 【초록키워드】 Molecular biology, Neutralizing antibodies, serology, antibody, SARS-COV-2 infection, Infection, immunoassay, RT-qPCR, cats, qPCR, one health, Comparative analysis, molecular, information, serological, microsphere, S-protein, observation, SARS-CoV-2 sequences, SARS-CoV-2 sequence, serological analysis, N-protein, symptomatic SARS-CoV-2 infection, variants of SARS-CoV-2, FIVE, evaluated, provide, 【제목키워드】 COVID-19 pandemic, detection, France, second wave,