Abstract
In the ongoing COVID-19 pandemic, rapid and sensitive diagnosis of viral infection is a critical deterrent to the spread of SARS-CoV-2. To this end, we developed an automated amplification-free digital RNA detection platform using CRISPR-Cas13a and microchamber device (opn-SATORI), which automatically completes a detection process from sample mixing to RNA quantification in clinical specimens within ~9 min. Using the optimal Cas13a enzyme and magnetic beads technology, opn-SATORI detected SARS-CoV-2 genomic RNA with a LoD of < 6.5 aM (3.9 copies μL -1 ), comparable to RT-qPCR. Additionally, opn-SATORI discriminated between SARS-CoV-2 variants of concern, including alpha, delta, and omicron, with 98% accuracy. Thus, opn-SATORI can serve as a rapid and convenient diagnostic platform for identifying several types of viral infections.
【초록키워드】 SARS-CoV-2, viral infection, COVID-19 pandemic, SARS-CoV-2 variant, diagnostic, Diagnosis, omicron, viral infections, RNA, Spread, CRISPR, RT-qPCR, Accuracy, SARS-CoV-2 variants, Alpha, automated, quantification, Critical, platform, genomic RNA, enzyme, specimen, magnetic bead, Complete, comparable, automatically, 【제목키워드】 SARS-CoV-2, Diagnosis, RNA, platform,