Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation-Specific PCR

Published on
Journal: Methods in molecular biology (Clifton, N.J.)
[저자] Yongmei Zhang, Richeng Mao, Haitao Guo, Jiming Zhang
[Category] B형 간염,
PMC URL    Pubmed URL    [DOI] 10.1007/978-1-4939-6700-1_7    
[Source] pubmed

Mapping of DNA methylation is essential in understanding the process of HBV covalently closed circular DNA (cccDNA) transcription. Here, bisulfite sequencing PCR and methylation-specific PCR, two PCR-based approaches used in determining and quantifying the DNA methylation pattern, are described.

All Keywords
【저자키워드】 DNA methylation, HBV cccDNA, Bisulfite sequencing PCR, Methylation-specific PCR.,