Abstract
The SARS-CoV-2 can be excreted in feces and can reach sewage systems. Determining the presence of infective viral particles in feces and sewage is necessary to take adequate control measures and to elucidate new routes of transmission. Here, we have developed a sample concentration methodology that allows us to maintain viral infectivity. Feces of COVID-19 patients and wastewater samples have been analyzed both by molecular methods and cell culture. Our results show no evidence of infective viral particles, suggesting that fecal-oral transmission is not a primary route. However, larger-scale efforts are needed, especially with the emergence of new viral variants.
Keywords: Fecal–oral transmission; SARS-CoV-2; COVID-19; Viral infectious particles; Wastewater.
【저자키워드】 COVID-19, SARS-CoV-2, wastewater, Fecal–oral transmission, Viral infectious particles, 【초록키워드】 Transmission, wastewater, viral infectivity, Cell culture, feces, Viral variants, sewage, methodology, molecular, Evidence, Concentration, Viral particles, Fecal–oral transmission, COVID-19 patient, evidence of, no evidence of, measure, effort, viral particle, infective, analyzed, maintain, Determining, Fece, 【제목키워드】 Evidence,