Abstract
A novel and sensitive voltammetric nanosensor was developed for the first time for trace level monitoring of favipiravir based on gold/silver core-shell nanoparticles (Au@Ag CSNPs) with conductive polymer poly (3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) and functionalized multi carbon nanotubes (F-MWCNTs) on a glassy carbon electrode (GCE). The formation of Au@Ag CSNPs/PEDOT:PSS/F-MWCNT composite was confirmed by various analytical techniques, including X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and field-emission scanning electron microscopy (SEM). Under the optimized conditions and at a typical working potential of + 1.23 V (vs. Ag/AgCl), the Au@Ag CSNPs/PEDOT:PSS/F-MWCNT/GCE revealed linear quantitative ranges from 0.005 to 0.009 and 0.009 to 1.95 µM with a limit of detection 0.46 nM (S/N = 3) with acceptable relative standard deviations (1.1-4.9 %) for pharmaceutical formulations, urine, and human plasma samples without applying any sample pretreatment (1.12-4.93%). The interference effect of antiviral drugs, biological compounds, and amino acids was negligible, and the sensing system demonstrated outstanding reproducibility, repeatability, stability, and reusability. The findings revealed that this assay strategy has promising applications in diagnosing FAV in clinical samples, which could be attributed to the large surface area on active sites and high conductivity of bimetallic nanocomposite.
Keywords: Biological sample analysis; COVID-19; Carbon nanotube; Conductive polymer; Favipiravir; Voltammetry.
【저자키워드】 COVID-19, Favipiravir, voltammetry, Biological sample analysis, Carbon nanotube, Conductive polymer, 【초록키워드】 repeatability, antiviral drugs, Favipiravir, Polymer, clinical samples, electron microscopy, stability, ultraviolet, Microscopy, limit of detection, amino acids, Urine, silver, transmission electron microscopy, compounds, Amino acid, SEM, Conductive polymer, Biological, active sites, active site, surface area, scanning electron microscopy, Standard deviation, X-ray diffraction, multi, reproducibility, diagnosing, carbon, TEM, human plasma samples, sulfonate, polystyrene sulfonate, AgCl, X-ray spectroscopy, linear, condition, demonstrated, human plasma sample, quantitative range, 【제목키워드】 Favipiravir, antiviral drug,