Abstract
Co-circulation of coronavirus disease 2019 (COVID-19) and dengue fever has been reported. Accurate and timely multiplex diagnosis is required to prevent future pandemics. Here, we developed an innovative microfluidic chip that enables a snapshot multiplex immunoassay for timely on-site response and offers unprecedented multiplexing capability with an operating procedure similar to that of lateral flow assays. An open microchannel assembly of individually engineered microbeads was developed to construct nine high-density test lines, which can be imaged in a 1 mm 2 field-of-view. Thus, simultaneous detection of multiple antibodies would be achievable in a single high-resolution snapshot. Next, we developed a novel pixel intensity-based imaging process to distinguish effective and non-specific fluorescence signals, thereby improving the reliability of this fluorescence-based immunoassay. Finally, the chip specifically identified and classified random combinations of arbovirus (Zika, dengue, and chikungunya viruses) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies within 30 min. Therefore, we believe that this snapshot multiplex immunoassay chip is a powerful diagnostic tool to control current and future pandemics.
Keywords: COVID-19; Immunoassay; Microfluidics; Multiplex assay; Rapid test.
【저자키워드】 COVID-19, Microfluidics, immunoassay, Multiplex assay, Rapid test, 【초록키워드】 coronavirus disease, viruses, SARS-CoV-2, Coronavirus disease 2019, coronavirus, reliability, antibody, diagnostic, Diagnosis, severe acute respiratory syndrome Coronavirus, Microfluidics, lateral flow, immunoassay, Dengue fever, Multiplex assay, Rapid test, Dengue, Pandemics, Rapid, Zika, multiplex, non-specific, arbovirus, Combination, High-resolution, Chikungunya, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, random, ChIP, offer, high-density, Prevent, effective, reported, assays, required, nine, 【제목키워드】 multiplex, platform, test line,