Abstract
Introduction: We describe cytologic and immunohistologic findings in virus transport medium on cases under investigation of SARS-CoV-2 infection.
Methods: Cytologic findings in cases under investigation of SARS-CoV-2 infection from one hundred consecutive nasopharyngeal swab were reviewed. Immunohistochemistry and SARSCoV-2 RT-PCR determination were performed to detect virus.
Results: No viral inclusions were noted in squamous cells obtained from virus transport medium. Immunohistochemical study with monoclonal antibody against SARS-CoV-2 viral nucleoprotein showed positivity in squamous cells. No positivity was present in others cellular components.
Conclusions: SARS-CoV-2 predominantly localizes squamous cells in cytology samples of patients with RT-PCR positive determination of SARSCoV-2. The results of the current study support the notion that the nasopharyngeal region is the anatomical station that SARS-CoV-2 infects first, and the infection can lead to the migration of the virus into the lower airways.
Keywords: COVID-19; RT-PCR; SARS-CoV-2; cytology; immunohistochemical; liquid-based; nasopharynx; swab; viral transport medium.
【저자키워드】 COVID-19, SARS-CoV-2, RT-PCR, nasopharynx, Swab, cytology, immunohistochemical, liquid-based, Viral transport medium, 【초록키워드】 immunohistochemistry, SARS-COV-2 infection, monoclonal antibody, Infection, RT-PCR, virus, Nasopharyngeal swab, Migration, Viral, cells, nasopharyngeal, Patient, nasopharynx, Swab, SARSCoV-2, cytology, lead, transport medium, squamous cells, Support, Viral transport medium, lower airways, positive, cellular components, virus transport medium, infect, Inclusion, Cell, SARS-CoV-2 viral, performed, anatomical, detect virus, 【제목키워드】 cytological,