Abstract
A rapid detection test for SARS-CoV-2 is urgently required to monitor virus spread and containment. Here, we describe a test that uses nanoprobes, which are gold nanoparticles functionalized with an aptamer specific to the spike membrane protein of SARS-CoV-2. An enzyme-linked immunosorbent assay confirms aptamer binding with the spike protein on gold surfaces. Protein recognition occurs by adding a coagulant, where nanoprobes with no bound protein agglomerate while those with sufficient bound protein do not. Using plasmon absorbance spectra, the nanoprobes detect 16 nM and higher concentrations of spike protein in phosphate-buffered saline. The time-varying light absorbance is examined at 540 nm to determine the critical coagulant concentration required to agglomerates the nanoprobes, which depends on the protein concentration. This approach detects 3540 genome copies/μl of inactivated SARS-CoV-2.
Keywords: Aptamer; Biosensing; Gold nanoparticle; SARS-CoV-2; Spike protein; Surface plasmon resonance.
【저자키워드】 SARS-CoV-2, Spike protein, aptamer, biosensing, Gold nanoparticle, Surface plasmon resonance., 【초록키워드】 spike, Genome, surface plasmon resonance, Spike protein, aptamer, Protein, enzyme-linked immunosorbent assay, membrane protein, gold, Critical, inactivated, binding, Gold nanoparticle, Containment, Concentration, phosphate, phosphate-buffered saline, surface plasmon, enzyme, virus spread, MONITOR, protein concentration, approach, enzyme-linked immunosorbent, coagulant, detect, examined, required, determine, occur, the spike protein, absorbance, light absorbance, 【제목키워드】 SARS-CoV-2 detection,