Abstract
Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is considered one of the worst pandemic outbreaks worldwide. This ongoing pandemic urgently requires rapid, accurate, and specific testing devices to detect the virus. We report a simple electrochemical biosensor based on a highly specific synthetic peptide to detect SARS-CoV-2 Spike protein. Unlike other reported electrochemical biosensors involving nanomaterials or complex approaches, our electrochemical platform uses screen-printed gold electrodes functionalized with the thiolated peptide, whose interaction with the Spike protein is directly followed by Electrochemical Impedance Spectroscopy. The electrochemical platform was Spike protein concentration-dependent, with high sensitivity and reproducibility and a limit of detection of 18.2 ng/mL when tested in Spike protein commercial solutions and 0.01 copies/mL in lysed SARS-CoV-2 particles. The label-free biosensor successfully detected the Spike protein in samples from infected patients straightforwardly in only 15 min. The simplicity of the proposed format combined with an on-demand designed peptide opens the path for detecting other pathogen-related antigens.
Keywords: Impedimetric biosensor; Infectious disease; SARS-CoV-2; Synthetic peptide.
【저자키워드】 SARS-CoV-2, Infectious disease, synthetic peptide., Impedimetric biosensor, 【초록키워드】 COVID-19, coronavirus disease, severe acute respiratory syndrome coronavirus 2, Coronavirus disease 2019, pandemic, spike, peptide, Infectious disease, severe acute respiratory syndrome Coronavirus, virus, coronavirus 2, Spike protein, Severe acute respiratory syndrome, Protein, sensitivity, Screen, outbreak, SARS-CoV-2 spike protein, limit of detection, gold, antigens, respiratory, nanomaterials, platform, synthetic, Interaction, impedance, followed by, Spectroscopy, acute respiratory syndrome coronavirus, infected patients, approaches, complex, simplicity, path, reproducibility, SARS-CoV-2 particles, tested, detect, caused, reported, infected patient, lysed, the Spike,