Abstract
The SARS-CoV-2 virus, which is driving the current COVID-19 epidemic, has been detected in wastewater and is being utilized as a surveillance tool to establish an early warning system to aid in the management and prevention of future pandemics. qPCR is the method usually used to detect SARS-CoV-2 in wastewater. There has been no study using an immunoassay that is less laboratory-intensive than qPCR with a shorter turnaround time. Therefore, we aimed to evaluate the performance of an automated chemiluminescence enzyme immunoassay (CLEIA) for SARS-CoV-2 antigen in wastewater. The CLEIA assay achieved 100% sensitivity and 66.7% specificity in a field-captured wastewater sample compared to the gold standard RT-qPCR. Our early findings suggest that the SARS-CoV-2 antigen can be identified in wastewater samples using an automated CLEIA, reducing the turnaround time and improving the performance of SARS-CoV-2 wastewater monitoring during the pandemic.
Keywords: CLEIA; COVID-19; RT-qPCR; SARS-CoV-2 antigen; wastewater.
【저자키워드】 COVID-19, RT-qPCR, wastewater, SARS-CoV-2 antigen, CLEIA, 【초록키워드】 SARS-CoV-2, pandemic, SARS-CoV-2 virus, Antigen, immunoassay, sensitivity, specificity, RT-qPCR, wastewater, Surveillance, qPCR, management, Pandemics, gold, automated, enzyme immunoassay, SARS-CoV-2 antigen, chemiluminescence, COVID-19 epidemic, enzyme, gold standard, driving, detect, evaluate, less, reducing, the SARS-CoV-2, 【제목키워드】 SARS-CoV2, detection,